Validated UV Method for the Determination of Chloroquine Sulphate and Its Stress Degradation Studies

The main objective of this paper was to develop a simple, sensitive and validated UV spectrophotometric method for the determination of chloroquine sulphate in pharmaceutical formulations. The aqueous solution of chloroquine sulphate absorbed maximally at 220 nm performed at room temperature (25 ± 1oC). Under optimized experimental conditions, Beer’s law was obeyed in the concentration range of 1.0 – 10.0 μg/ml. The method was validated with respect to accuracy, precision, limits of detection and quantitation. The drug was also subjected to acid and alkaline hydrolysis, photolysis, thermal and degradation with time to determine the stability of a drug substance in pure and pharmaceutical formulations. Robustness testing was also conducted to evaluate the effect of minor changes to the absorbance of pharmaceutical formulations.